ఇంటర్నేషనల్ జర్నల్ ఆఫ్ రీసెర్చ్ అండ్ డెవలప్‌మెంట్ ఇన్ ఫార్మసీ & లైఫ్ సైన్సెస్

నైరూప్య

SIMULTANEOUS ESTIMATION OF CLOTRIMAZOLE AND TINIDAZOLE IN A TABLET USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Patel S K, Kapupara P P, Shah K V

A Simple, efficient and reproducible method for the simultaneous determination of Clotrimazole and Tinidazole from pharmaceutical formulation has been developed using reversed phase high performance liquid chromatography. The separation was carried out using a mobile phase consisting of methanol :
acetonitrile (95:5 % V/V). Column used was chromacil C18 (250 X 4 mm internal diameter) 5 μ with flow rate of 1 ml/min. The detection wavelength used was 229nm. The mean retention time of Clotrimazole and Tinidazole were 6.84 min and 5.54 min respectively. Linearity of both drugs are 10-50 μg/ml. Analytical
parameters were calculated and a full statistical evaluation included.