ఇంటర్నేషనల్ జర్నల్ ఆఫ్ రీసెర్చ్ అండ్ డెవలప్‌మెంట్ ఇన్ ఫార్మసీ & లైఫ్ సైన్సెస్

నైరూప్య

QUERCETIN IN CALLUS CULTURES OF CLITORIA TERNATEA LINN

Rasheeduz Zafar, Parisa Humayun

Callus cultures of leaf, root and hypocotyledon of Clitoria ternatea were developed independently in MS medium supplemented with 1mgl-1 each of 2,4- dichlorophenoxy acetic acid (2,4-D), benzyl adenine (BA), Indole-3-acetic acid (IAA), and Kinetin. Three months old calli were subjected to phytochemical screening which showed the presence of alkaloids, amino acids, flavonoids, carbohydrates, phenolic compounds, tannins, saponins, mucilage and proteins. Quercetin, a flavonoid having pharmacological functions was quantitatively estimated by Reversed – Phase HPLC in natural leaf and callus cultures of leaf, hypocotyledon and root. Leaf callus was found to content the highest amount of Quercetin, i.e. 1.21% w/v as compared to other callus while natural leaf extract showed 1.25%w/v of Quercetin.