బయోరేమిడియేషన్ & బయోడిగ్రేడేషన్ జర్నల్

నైరూప్య

Production and Qualitative Analysis of Biosurfactant and Biodegradation of the Organophosphate by Nocardia mediterranie

Sukirtha TH and Usharani MV

Synthetic Organophosphorus (OP) compounds are used extensively as agricultural and domestic pesticides and could be used as chemical warfare agents. The degradation of organophosphates in soil has gained a lot of importance due to increase the quantities of its use all over the world. The biodegradation of organophosphates were carried out by bacteria, fungi and algae effectively. The biodegradation of organophosphates in flask-quoted conditions was studied by an isolated Nocardia mediterranei from the soil under laboratory condition. The biodegradation process was carried out by the enrichment technique. The biodegradation rate by Nocardia mediterranei enhanced the rate of biodegradation to 20%–30%. Addition of the biosurfactant produced from the Nocardia mediterranei enhanced the rate of biodegradation to 30%–45%. The produced biosurfactant was found to be specific to organophosphates. The TLC technique is used for the identification of glycolipid compound in the biosurfactant. Identification and quantification analysis of biosurfactant were carried out through Standard by HPLC (Column 18). The chemical structure of the biosurfactant was identified as a glycolipid called Trehalolipid by GC-MS.