బయోటెక్నాలజీ & బయోమెటీరియల్స్

నైరూప్య

Preparation of Emulsified Encapsulated Nanoparticles of Bovine Serum Albumin of Bound Glucose Oxidase and their Application in Soft Drinks/ Non-Alcoholic Beverage

Kirti Rani Sharma

Microspheres are nanoparticles in which the glucose oxidase was encapsulated by emulsification and immobilized through covalent coupling by glutaraldehyde which is an excellent cross linking agent. Biodegradation of these emulsified encapsulated nanoparticles of bovine serum albumin of bound glucose oxidase was carried out by the proteases such as trypsin, chymotrypsin and papain. Chymotrypsin was found to be the best proteolytic enzyme among these proteases. These nanoparticles of bovine serum albumin of bound glucose oxidase were converted the glucose into gluconic acid and hydrogen peroxide in the delivery system which in turn minimized the utilization of the excess of glucose by the microbes/pathogens as a substrate for their growth. Coca-Cola has approximately half of the world market share and called as industrial player. And, then Pepsi sales stood at US $36 billion per market capitalization and thus the value sales of soft drinks/ non-alcoholic beverages will increase 27% by 2014 worldwide. Glucose is the major content of these drinks. Further, these glucose oxidase bound emulsified nanoparticles of bovine serum albumin along with chymotrypsin were used in soft drinks/non-alcoholic beverages which made the drinks free from turbidity and opalescence as well as to maintain the pH as acid regulator.