బయోరేమిడియేషన్ & బయోడిగ్రేడేషన్ జర్నల్

నైరూప్య

Mycoremediation of Imidaclopridin the Presence of Different Soil Amendments using Trichoderma_longibrachiatum and Aspergillusoryzae Isolated from Pesticide Contaminated Agricultural fields of Uttarakhand

Saurabh Gangola, Pankaj, Priyanka Khati and Anita Sharma

Imidacloprid (I-[(6-chloro-3-pyridinyl)-methyl]-N-nitro-2-imidazolidinimine), is a chloronicotinyl insecticide and used to control biting and sucking insects, It is very persistent in the soil with a half-life of more than 100 days. Biodegradation of imidacloprid (20) ppm was checked at 0, 10 and 15 day interval by two fungal isolates, from contaminated soil of agricultural field. The isoates were characterized as Aspergillusoryzae and Trichodermalongibrachiatumon the basis of morphological and molecular techniques. Highest degradation of imidacloprid was reported in consortium (92%) followed by FII(85%), FIII (81%) and control(11%)after 15 days.Immobilization of fungal isolates in sodium alginate and agar disc enhanced biodegradation of imidacloprid. After 15 days, maximum biodegradation of imidacloprid was (95%) and (97%) in consortium immobilized in sodium alginate, and agar discs respectively. Effect of organic amendment on rate of biodegradation was also observed. The maximum biodegradation was observed in consortium amended with bagasse (99%) followed by consortium amended with Hen Manure (94%) and consortium amended with Farm Yard Manure (91%). ITS regions of the two fungal isolates i.e FII and FIII showed 100% similarity with Aspergillusoryzae and Trichodermalongibrachiatum respectively.