బయోరేమిడియేషన్ & బయోడిగ్రేడేషన్ జర్నల్

నైరూప్య

Monitoring of Molecular Variation among Egyptian Faba Bean Rhizobium Isolates as Response to Pesticides Stress

Khalid S. Abdel-lateif, Abdel Fattah M. El-Zanaty, Omar A. Hewedy and Mohamed A. El Sobky

Ten Rhizobium isolates from nodules of Faba bean (Vicia faba L.) plants that represent different geographic and climatic locations in Egypt were evaluated for their tolerance against different doses of Zeus and Stomp as pesticides (250, 500 and 750 mg). In general, with increasing of pesticides doses, the tolerance and the viability of isolates were decreased. All isolates did not succeed to grow at the highest dose of Zeus (750 mg), while the isolates RLA, RLG and RLI succeeded to grow at the same concentration of Stomp. The most important nodulation genes nodA, nodD and the bidirectional promoter region between them were amplified using PCR and two principals bands were detected in the most isolates. The protein banding patterns of some Rhizobium isolates were detected using SDSPAGE analysis under pesticides stress and compared with their untreated control.