బయోరేమిడియేషన్ & బయోడిగ్రేడేషన్ జర్నల్

నైరూప్య

Isolation, Screening and Identification of Soil Bacteria Capable of Degrading Pesticide

Sameer Patil* , M. B. Doddamani, Mahadev Khetagoudar, Giridhar Goudar

The yield of the crops is facing decline due to harmful insects, small animals, wild plants, and other unwanted organisms. To minimize this parameter farmers are spraying chemical substance called as pesticide. Its utilization in large scale leads to contamination of the soil biosphere which leads to damage of the public health. Bioremediation technique is used for controlling hazardous effect of pesticides. It is a technique utilized to control the pollutants by the microorganisms. Keeping these parameters pesticide contaminated soil samples were collected and analyzed from different locations of Agriculture University Dharwad campus (Dharwad District). Two bacterial isolates were obtained by enrichment and isolation methods by using agar medium with specific 2, 4 DDT and Dicofol pesticides. The soil samples were screened for its conductivity, pH and Dehydrogenase activity. The pesticide tolerance capacity in solid media and sequencing was done to identify the isolate. The isolates were identified as Leclercia adecarboxylata and Pseudomonas aeruginosa through sequence similarity search. Effect of pH and temperature on growth of cultures in presence of pesticides showed optimum growth at pH 6 and pH 5. Conductivity for soil samples varied from 0.2 to 1.6 S/m. However, both the cultures show optimum growth at 30°C.