జర్నల్ ఆఫ్ ఇన్ఫెక్షియస్ డిసీజెస్ అండ్ థెరపీ

నైరూప్య

Identification and Biotyping of Escherichia coli from Diarrheic Lambs in and Around Debrebirhan Town, Ethiopia

Metasebia Aklilu, Tesfaye Sisay, GeneneTefera and Belay Tekalign

Background: Infectious diarrhea is the most significant cause of morbidity and mortality in neonatal dairy sheep throughout the world. This is the first study conducted on isolation and bio typing of E. coli from lamb diarrhea in and around DebreBirhan. The present study was undertaken with the objective of isolation, omnilog characterization and biotyping of E. coli isolates from faecal samples around Debrebrhan.

Methods: A cross-sectional study was conducted from October, 2012 to April, 2013. The study focused on lambs less than three months of age showing clinical symptoms of diarrhea. Standard cultural and Omnilog tests were done to identify E. coli species and biotypes; descriptive statistics and Chi-square were used to analyze the collected data.

Results: From a total of 100 diarrheic lambs examined, 84% were found to be positive for E. coli. On the basis of fermentation reactions of sugars, viz. dulcitol, raffinose, rhamnose, salicin, starch and sucrose, 69 isolates utilized one or more sugar, while 14 isolates failed to utilize any of the sugars. 69 E. coli isolates were bio typed into 15 different combinations. Association between different age groups and occurrence of lamb diarrhea caused by E. coli strains showed a significant association (p<0.05). From the questionnaire findings, 60% of all the health problems in lambs were due to diarrhea where young sheep were more affected.