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నైరూప్య

DNA Fingerprinting of Rice Lines for Salinity Tolerance at Reproductive Stage

Iqbal SA, Mirza Mofazzal Islam, Ahmed Hossain Md and Ananya Malaker

Salinity is the most common abiotic stresses leading to the reduction of rice yield in many rice-growing areas of the world. This study was undertaken to assess the genetic diversity among saline treatment and susceptible rice lines using molecular marker (SSR). Salinity screening was performed at reproductive stage using sustained water bath following IRRI standard protocol. Twenty two rice lines were used for molecular analysis using three SSR markers: RM1287, RM342 and RM493 to determine salinity tolerance at reproductive stage. For DNA fingerprinting of rice Varieties, DNA was extracted from leaf samples using IRRI standard protocol. Amplified microsatellite loci were analyzed for polymorphism using Polyacrylamide Gel Electrophoresis (PAGE) and the result revealed that all the primers detected polymorphism among the rice lines analyzed. Using 3 SSR markers, a total of 25 alleles were detected among the 22 rice lines. The polymorphism information content (PIC) reflects the diversity allele frequency among the lines, which ranged from 0.59 to 0.88 with an average of 0.74. RM493 was the best marker for identification of genotypes as revealed by PIC values. The results of microsatellite marker based DNA fingerprinting analysis will be useful for the selection of parents for developing salt tolerant rice variety through molecular breeding.