బయోరేమిడియేషన్ & బయోడిగ్రేడేషన్ జర్నల్

నైరూప్య

Bioremoval of Azo dye Reactive Red by Bacillus spp. ETL-1982

Maulin P Shah, Patel KA, Nair SS and Darji AM

Bacillus spp. ETL-1982, with notable ability to decolorize the reactive textile dye Reactive Red, was isolated from the activated sludge collected from a textile mill. Phenotypic characterization and phylogenetic analysis of the 16S rDNA sequence indicated that the bacterial strain belonged to the genus Bacillus. Bacillus ETL-1982 showed a strong ability to decolorize reactive textile dye at pH 9.0 and 37°C and with 4 gL-1 glucose concentrations was considered to be the optimum decolorizing conditions. Bacillus spp. ETL-1982 grew well in a high concentration of dye (300 mgl-1), resulting in approximately 96% decolorization extent in 36 h, and could tolerate up to 1000 mg l-1 of dye. UV–Vis analyses and colorless bacterial cells suggested that Bacillus spp. ETL-1982 exhibited decolorizing activity through biodegradation, rather than inactive surface adsorption. High decolorization extent and facile conditions show the potential for this Bacillus ETL-1982 to be used in the biological treatment of dyeing mill effluents.