బయోరేమిడియేషన్ & బయోడిగ్రేడేషన్ జర్నల్

నైరూప్య

Bioremediation on Effluents from Magnesite and Bauxite Mines using Thiobacillus Spp and Pseudomonas Spp

Narayanan Mathiyazhagan and Devarajan Natarajan

The present investigation was carried out to isolate metal tolerant bacterial species from the waste dump of magnesite and bauxite mines, Salem district, Tamil nadu. The result of primary and secondary screening showed two basophilic microbes were isolated from the effluents of mining industry. These two bacterial spp (namely P. aeruginosa and T. ferrooxidans ) were adapted for metal tolerance test, with different concentrations of (20 to 100μg/ ml -1 ) heavy metals (Zn, Mn, Cu, Cr and Hg). The P. aeruginosa and T. ferrooxidans showed high resistant to all metals. The minimum inhibitory concentration (MIC) of T. ferrooxidans tolerate upto 200 μg/ml -1 and P. aeruginosa posses MIC up to 150 μg/ml -1 concentration of heavy metals in order of Mn > Fe > Cu > Cr > Hg. These bacteria also expressed some antibiotic resistant potentiality was calculated by Antibiotic Resistant Index (ARI). The pre and post treated soils were analyzed by Atomic Absorbance Spectrophotometer (AAS) for heavy metals. The results of biosorption process showed the T . ferrooxidans reduced/ absorbed some heavy metals from mines (Cd, Ca, Zn, Cr, Mn & Pb) and P. aeruginosa was effectively absorbing the most of the metals than T. ferrooxidans . Both species are effectively absorbing Cd, Ca, Zn followed by Pb. This study include role of pH are also discussed. This study will be encourages further toxic metals remediation program and safe the environment in future.