జర్నల్ ఆఫ్ ఎనలిటికల్ & బయోఅనలిటికల్ టెక్నిక్స్

నైరూప్య

Advanced LC-MS/MS Techniques Dissecting Diverse Isomers of Plant Sphingolipid Species

Toshiki Ishikawa, Daiki Yanagawa, Maki Kawai-Yamada and Hiroyuki Imai

Sphingolipid is a major lipid class ubiquitously present in eukaryotes and several species of prokaryotes. Recent genetic and sphingolipidomic researches have been revealing complex structures of plant sphingolipids and their functions in various aspects of plant biological events as major membrane components as well as signaling molecules. Particularly, cis/transisomeric double bond at Δ8 position of long-chain bases (LCBs) is of great interest due to their potential impacts on stress tolerance in plants and yeast. In addition, free LCBs and their metabolism are also important for intracellular signaling pathways during various environmental stresses. In this review, we introduce two current improvements of sphingolipidomic analyses based on liquid chromatography-tandem mass spectrometry (LC-MS/MS), focusing on high-throughput quantitative analysis of complex structural isomers of plant sphingolipids.